{"id":2697,"date":"2024-06-24T14:11:07","date_gmt":"2024-06-24T14:11:07","guid":{"rendered":"https:\/\/www.vanderbilt.edu\/evolution\/?p=2697"},"modified":"2024-10-01T14:58:00","modified_gmt":"2024-10-01T14:58:00","slug":"vu-graduate-student-explores-ancient-oral-microbiomes","status":"publish","type":"post","link":"https:\/\/www.vanderbilt.edu\/evolution\/vu-graduate-student-explores-ancient-oral-microbiomes\/","title":{"rendered":"VU Graduate Student Explores Ancient Oral Microbiomes"},"content":{"rendered":"

By Neomi Chen, Evolutionary Studies undergraduate communications assistant<\/em><\/p>\n

As Katie McCormack meticulously extracted calcified dental plaque from ancient Peruvian teeth, she felt the weight of centuries of history in her hands. This intricate work, part of her groundbreaking research in the Jada Benn Torres Lab<\/a>, aims to unravel the secrets of ancient oral health. With the support of the Evolutionary Studies Initiative<\/a> (ESI) and a recent 国产原创 Dissertation Enhancement Grant, McCormack has made significant strides in her study of ancient pathogens and their impact on human evolution.<\/p>\n

Oral Microbiome Project at La Real Site in the Yungas Zone of the Peruvian Andes<\/strong><\/p>\n

Data Collection<\/em><\/p>\n

\""Two
McCormack with Dr. Benn Torres in Tyvek suits at the University of Oklahoma ancient DNA lab.<\/figcaption><\/figure>\n

In December 2021, McCormack began removing microbial DNA from five sets of archaeological teeth to screen for respiratory and oral pathogens and study the oral microbiome of prehistoric humans\u2014early humans who lived before recorded history. In the ancient DNA lab at the University of Oklahoma (OU), McCormack suited up in full Tyvek gear, a stark reminder of the precautions necessary to handle precious, degraded samples. She received oral microbiome paleogenomics training from Dr. Tanvi Honap who worked in the Laboratories of Molecular Anthropology and Microbiome Research<\/a> headed by Dr. Cecil Lewis.<\/span><\/p>\n

Preliminary Results<\/em><\/p>\n

When McCormack received the genomic sequencing back from OU, she embarked on the initial taxonomic classification stage. By comparing the DNA of the oral pathogens to known databases, MetaPhlAn4 and KrakenUniq determined the identities of these pathogens, allowing McCormack to gain a deeper understanding of the microbial communities. The presence of Red Complex pathogens in three individuals\u2013\u2013Tannerella forsythia<\/em>, Treponema denticola<\/em>, and Porphyromonas gingivalis<\/em>\u2013\u2013sheds new light on the prevalence of periodontal and endodontic disease in ancient Andean communities. Due to changes in diet around that time, McCormack highlighted the potential association between periodontal diseases and maize consumption in South America. In April 2024, McCormack presented this preliminary data and findings at the Society for American Archaeology<\/a> conference.<\/span><\/p>\n

Next Steps<\/em><\/p>\n

As McCormack riffled through the metagenomic data, she found that one of the individuals had evidence of tuberculosis (TB). That discovery propelled her to use her recently awarded 国产原创 Dissertation Enhancement Grant to collaborate with Anne Stones\u2019 lab at Arizona State University<\/a> and her graduate student Adele Crane for the capture work and target enrichment of <\/span>mycobacterium tuberculosis<\/em>\u2013\u2013a complex of closely related bacteria that causes TB. \u201cTuberculosis has co-evolved with humans for millions of years,\u201d McCormack explained, emphasizing the disease\u2019s deep-rooted presence in our evolutionary history. By adopting an infectious disease standpoint, McCormack aims to better understand the phylogenetic relationships within the TB genome.<\/span><\/p>\n

Evolutionary Studies Initiative Outreach<\/strong><\/p>\n

At High Schools<\/em><\/p>\n

\""A
McCormack explaining a phylogenetic tree to a roomful of high school students in Nashville.<\/figcaption><\/figure>\n

For the last three years, McCormack has been involved with several ESI outreach projects such as giving brief lectures about human evolution at local high schools. McCormack reflected on her experience conveying the significance of evolution to a different student pool: \u201cI\u2019m used to TA-ing in undergrad courses and enjoy teaching at the college level, but working with high schoolers is a lot of fun because they have very different understandings of science.\u201d During a workshop at a Nashville high school, students eagerly compared their own features to a full-scale cast of Lucy, one of the earliest known hominids. \u201cSeeing their excitement was incredibly rewarding,\u201d McCormack recalled.<\/p>\n

At Coon Creek Science Center in West Tennessee<\/em><\/p>\n

Having attended the ESI field trip to Coon Creek Science Center for two consecutive years, McCormack has enjoyed hunting for fossils with other ESI team members and high school students. \u201cPaleontology is very much not my specific discipline, but it\u2019s a big science interest and hobby of mine to do with others,\u201d McCormack shared, highlighting her overall greater passion for the sciences.<\/p>\n","protected":false},"excerpt":{"rendered":"

By Neomi Chen, Evolutionary Studies undergraduate communications assistant As Katie McCormack meticulously extracted calcified dental plaque from ancient Peruvian teeth, she felt the weight of centuries of history in her hands. This intricate work, part of her groundbreaking research in the Jada Benn Torres Lab, aims to unravel the secrets of ancient oral health. With…<\/p>\n","protected":false},"author":2421,"featured_media":2698,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"spay_email":"","jetpack_publicize_message":"","jetpack_is_tweetstorm":false,"jetpack_publicize_feature_enabled":true},"categories":[1],"tags":[58,13,14,15,117],"acf":[],"yoast_head":"\n\n\n\n\n\n\n\n\n\n\n\n\t\n\t\n\t\n\n\n\t\n\t\n\t\n